Journal: International journal of molecular sciences

Article Title: Modulation of the Cardiovascular Risk in Type 1 Diabetic Rats by Endurance Training in Combination with the Prebiotic Xylooligosaccharide.

doi: 10.3390/ijms251810027

Figure Lengend Snippet: Figure 4. Study design. BP—blood pressure, HR—heart rate, MTE—maximum time to ex- haustion, AC—abdominal circumference, T-AOC—total antioxidant capacity, Il-6—interleukin-6, hsCRP—high-sensitive C-reactive protein, NADH + H+—nicotinamide adenine dinucleotide hydride, CoQ—coenzyme Q, LDH—lactate dehydrogenase, SDH—succinate dehydrogenase.

Article Snippet: Serum T-AOC, IL-6 and hsCRP were analyzed on an enzyme-linked immunosorbent assay (ELISA) microplate reader HumanReader.HS, HUMAN (Wiesbaden, Germany) via commercially available kits [Rat Total antioxidant capacity, T-AOC Elisa kit, Nanjing Pars Biochem CO., Ltd., Nanjing, China; Rat IL-6 (Interleukin 6) ELISA Kit, Elabscience Biotechnology Inc., Houston, TX, USA; Rat hsCRP (high-sensitivity, C-Reactive Protein) Elabscience Biotechnology Inc., Houston, TX, USA].

Techniques:

Journal: ImmunoTargets and Therapy

Article Title: Recombinant Antithrombin Alleviated Pulmonary Injury and Inflammation in LPS-Induced ARDS by Inhibiting IL17a/NF-κB Signaling

doi: 10.2147/ITT.S502925

Figure Lengend Snippet: rAT reduced pulmonary inflammation in LPS-induced ARDS model mice. ( A–D ) The levels of inflammatory factors, including IL-6 ( A ), TNF-α ( B ), IL-8 ( C ), and hs-CRP ( D ), were decreased in the serum of the rAT-treated group, as detected by ELISA. ( E ) Immunohistochemical staining for F4/80 revealed that rAT treatment reduced the proportion of macrophages in the lung tissue of LPS-induced ARDS model mice. ( F ) Immunohistochemical staining for MRCI, a marker of M2 macrophages, showed that rAT treatment increased the proportion of M2 macrophages in the lung tissue of LPS-induced ARDS model mice. ( G ) Immunohistochemical staining for Ly6G, a marker of neutrophils, showed that rAT treatment reduced the proportion of neutrophils in the lung tissue of LPS-induced ARDS model mice. All the data are presented as the means ± SDs of three independent experiments. One-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant; scale bar, 50 μm.

Article Snippet: A mouse CXCL15 ELISA Kit (E-EL-M0269) and a mouse hs-CRP ELISA Kit (E-EL-M0677) were purchased from Elabscience (Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Staining, Marker

Journal: ImmunoTargets and Therapy

Article Title: Recombinant Antithrombin Alleviated Pulmonary Injury and Inflammation in LPS-Induced ARDS by Inhibiting IL17a/NF-κB Signaling

doi: 10.2147/ITT.S502925

Figure Lengend Snippet: The efficacy of rAT in mitigating lung injury, suppressing the immune response, and inhibiting the activation of the NF-κB signaling pathway in LPS-induced ARDS mice were diminished by the administration of IL-17a. ( A ) ELISA results demonstrated that the administration of IL17a inhibited the ability of rAT to reduce inflammatory factors, including IL-6, TNF-α, and IL-8, in the serum of LPS-induced ARDS mice. ( B ) The analysis of the wet/dry weight ratio of the lung tissue revealed that the administration of IL17a counteracted the ability of rAT to alleviate pulmonary exudation in LPS-induced ARDS mice. ( C ) The administration of IL17a did not significantly affect the ability of rAT to reduce the number of cells in the BALF of LPS-induced ARDS mice. ( D ) The administration of IL17a attenuated the ability of rAT to reduce the concentrations of proteins in the BALF of LPS-induced ARDS mice. ( E ) Real-time PCR results showed that the administration of IL17a blocked the ability of rAT to downregulate the expression of target genes in the IL17a/NF-κB signaling pathway. ( F ) The protein levels of the NF-κB signaling pathway were assessed by Western blotting, and gray intensity analysis of the blots showed that the administration of IL17a in LPS-induced ARDS mice counteracted the ability of rAT to suppress the phosphorylation of IκBα, IKKα/β, and P65. The data are expressed as the means ± SDs (n=3 in each group). One-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001, and ns not significant.

Article Snippet: A mouse CXCL15 ELISA Kit (E-EL-M0269) and a mouse hs-CRP ELISA Kit (E-EL-M0677) were purchased from Elabscience (Wuhan, China).

Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Phospho-proteomics

Journal: International Journal of Molecular Sciences

Article Title: Association Between Pentraxins and Obesity in Prediabetes and Newly Diagnosed Type 2 Diabetes Mellitus Patients

doi: 10.3390/ijms26083661

Figure Lengend Snippet: Comparison between the studied groups as regards inflammatory biomarkers.

Article Snippet: For each of the following mediators, we used commercially available test sets: PTX3 (Catalog# E-EL-H6081; sensitivity: 4.12 pg/mL; detection range: 6.86–5000 pg/mL), hs-CRP (Catalog# E-EL-H5134; sensitivity: 9.38 pg/mL; detection range: 15.63–1000 pg/mL), from the Elabscience (Houston, TX, USA); TNF-α (Catalog# BMS223-4; sensitivity: 2.3 pg/mL; assay range: 7.8–500 pg/mL), IL-6 (Catalog# BMS213-2; sensitivity: 0.92 pg/mL; assay range: 1.56–100 pg/mL), from Invitrogen, Thermo Fisher Scientific, Inc. (Waltham, MA, USA).

Techniques: Comparison

Journal: International Journal of Molecular Sciences

Article Title: Association Between Pentraxins and Obesity in Prediabetes and Newly Diagnosed Type 2 Diabetes Mellitus Patients

doi: 10.3390/ijms26083661

Figure Lengend Snippet: Evaluation of Parameters According to the BMI Categories in the Studied Groups.

Article Snippet: For each of the following mediators, we used commercially available test sets: PTX3 (Catalog# E-EL-H6081; sensitivity: 4.12 pg/mL; detection range: 6.86–5000 pg/mL), hs-CRP (Catalog# E-EL-H5134; sensitivity: 9.38 pg/mL; detection range: 15.63–1000 pg/mL), from the Elabscience (Houston, TX, USA); TNF-α (Catalog# BMS223-4; sensitivity: 2.3 pg/mL; assay range: 7.8–500 pg/mL), IL-6 (Catalog# BMS213-2; sensitivity: 0.92 pg/mL; assay range: 1.56–100 pg/mL), from Invitrogen, Thermo Fisher Scientific, Inc. (Waltham, MA, USA).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Association Between Pentraxins and Obesity in Prediabetes and Newly Diagnosed Type 2 Diabetes Mellitus Patients

doi: 10.3390/ijms26083661

Figure Lengend Snippet: Comparing the Inflammatory Biomarkers Values Between the HbA1c Quartiles in the PreDM Group.

Article Snippet: For each of the following mediators, we used commercially available test sets: PTX3 (Catalog# E-EL-H6081; sensitivity: 4.12 pg/mL; detection range: 6.86–5000 pg/mL), hs-CRP (Catalog# E-EL-H5134; sensitivity: 9.38 pg/mL; detection range: 15.63–1000 pg/mL), from the Elabscience (Houston, TX, USA); TNF-α (Catalog# BMS223-4; sensitivity: 2.3 pg/mL; assay range: 7.8–500 pg/mL), IL-6 (Catalog# BMS213-2; sensitivity: 0.92 pg/mL; assay range: 1.56–100 pg/mL), from Invitrogen, Thermo Fisher Scientific, Inc. (Waltham, MA, USA).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Association Between Pentraxins and Obesity in Prediabetes and Newly Diagnosed Type 2 Diabetes Mellitus Patients

doi: 10.3390/ijms26083661

Figure Lengend Snippet: Comparing the Inflammatory Biomarkers Values Between the HbA1c Quartiles in the T2DM Group.

Article Snippet: For each of the following mediators, we used commercially available test sets: PTX3 (Catalog# E-EL-H6081; sensitivity: 4.12 pg/mL; detection range: 6.86–5000 pg/mL), hs-CRP (Catalog# E-EL-H5134; sensitivity: 9.38 pg/mL; detection range: 15.63–1000 pg/mL), from the Elabscience (Houston, TX, USA); TNF-α (Catalog# BMS223-4; sensitivity: 2.3 pg/mL; assay range: 7.8–500 pg/mL), IL-6 (Catalog# BMS213-2; sensitivity: 0.92 pg/mL; assay range: 1.56–100 pg/mL), from Invitrogen, Thermo Fisher Scientific, Inc. (Waltham, MA, USA).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Association Between Pentraxins and Obesity in Prediabetes and Newly Diagnosed Type 2 Diabetes Mellitus Patients

doi: 10.3390/ijms26083661

Figure Lengend Snippet: Diagnostic performance of the investigated parameters.

Article Snippet: For each of the following mediators, we used commercially available test sets: PTX3 (Catalog# E-EL-H6081; sensitivity: 4.12 pg/mL; detection range: 6.86–5000 pg/mL), hs-CRP (Catalog# E-EL-H5134; sensitivity: 9.38 pg/mL; detection range: 15.63–1000 pg/mL), from the Elabscience (Houston, TX, USA); TNF-α (Catalog# BMS223-4; sensitivity: 2.3 pg/mL; assay range: 7.8–500 pg/mL), IL-6 (Catalog# BMS213-2; sensitivity: 0.92 pg/mL; assay range: 1.56–100 pg/mL), from Invitrogen, Thermo Fisher Scientific, Inc. (Waltham, MA, USA).

Techniques: Diagnostic Assay